RPPA

RPPA

Reverse-phase protein arrays (RPPA) offer great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening using cultured cell systems.

In this course, we represent a basic molecular biology background that will be able to demonstrate conceptual knowledge and technical skills for using protein micro-arrays as a research study upon completion of an intensive 2-wk training course with lecture and laboratory practical work per week. In addition, we will explain the use of porous nitrocellulose (NC) film slides as a preferred substrate for RPPA due to their high protein binding capacity and many functional advantages. Additionally, the compatibility of NC with mass spectrometry allows for the extension of RPPA technology with emerging downstream protein characterization methodologies.

Upon completion of this course, the participants will ba able to construct the following:

  1. Design laboratory experiments to target dominant drivers of the cellular phenotype that is the consequence of the genomic lesion.
  2. Distinguish the active state of protein signal pathways, and protein-protein interactions represent a rich source for new molecular targeted therapeutics.
  3. Formulate and examine the quantitative levels of cell surface receptors targeted by immune checkpoint inhibitors.
  4. Observe the limitations of qualitative and quantitative assessment techniques.
  5. Underline and define the primary literature with updated information.

Researchers, near-graduation and graduate students in the areas of Biological and Medical Sciences.