Western Blot

Western Blot

The basic concepts of Western blot

Since the development of the protein analysis technique, the western blot technique became one of the gold standards in protein analysis.

Western blots enable investigators to determine the molecular weight of a protein measure the relative amounts of the proteins presented in different tissue samples.

Although with the advancement of molecular biology techniques, most bioscience researchers still heavily rely on the western blot technique for protein analysis.

Since most of the research required skills in western blot at some point in their careers, this course is designed to offer the trainees the fundamental aspects of protein analysis through the western blot.

Additionally, the course will deliver the essential knowledge in choosing the appropriate primary and secondary antibodies.

Moreover, the course is offering the students the opportunity to learn the most up-to-date molecular techniques as the trainees will learn how to design and perform their own experiments and then evaluate the results. The trainees will also be guided through a detailed fluorescent western blot protocol and share some troubleshooting tips.

The attendees will have deep knowledge of the current and future aspects of the applications of the western blot which will advance their career in molecular biology.

By completing the course, the attendee will have a background on the western blot technique and be able to conclude the concept of:

  1. Demonstrate the basics of the protein extraction techniques
  2. Evaluate the basic principles of western blot analysis
  3. Choosing the right antibodies for your western blot experiment
  4. Appling the appropriate controls for western blot analysis
  5. Assess the advantages of fluorescence vs ECL
  6. Demonstrating fluorescent western blot protocol
  7. Categorizing the troubleshooting the most common problems in western blot

Basic University training in Biological and Medical Sciences