Enzyme Linked Immunosorbent Assay (ELISA)
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Fundamental aspects on the Enzyme linked immunosorbent assay (ELISA)
Who is this course for?
Researchers, near-graduation and graduate students in the areas of Biological and Medical Sciences
Enzyme-linked immunosorbent assay (ELISA) is a widely established technology used to detect the presence of antigens in samples. Protein analysis has become the center of the innovation of molecular biology research. The history of protein analysis in relation to medical discoveries is one of the major breakthroughs in the 21st century from which patients and families have benefited hugely. In this ELISA course, we will highlight the importance of ELISA techniques along with their principles. The trainees will be introduced to the possibilities of ELISA techniques in theory and practice, and they will develop the competence needed to establish these techniques in the laboratory. The theoretical module covers the possibilities and parameters of different methods while in the practical part, they will use this theoretical background to analyze proteins. In order to conduct the ELISA, the attendees will independently establish an ELISA system and optimize it with little assistance. Therefore, the trainees will have deep knowledge of the current and future aspects of the applications of ELISA.
By the end of this course, the participants will be able to determine the concept of:
- State the ELISA techniques and how important is it?
- Underline the advantages and disadvantages of the ELISA techniques
- Assess the ELISA principles
- Observe different types of ELISA
- Design ELISA assay formats
- Define the different components of ELISA systems
- Assemble ELISA antibody conjugates
- Demonstrate the possibilities for optimization of components
- Evaluate of optimal ELISA concepts
- Interpret the optimization of robustness and stability of the test system
- Create strategy from assessment of demand to the final assay
- Conclude of capacity verification and validation of an ELISA
- Justify trouble-shooting by systematic error analysis
- Formulate ELISA standardization
Basic University training in Biological and Medical Sciences.